Modelling DNA stretching for physics and biology
Identifieur interne : 000265 ( France/Analysis ); précédent : 000264; suivant : 000266Modelling DNA stretching for physics and biology
Auteurs : Richard Lavery [France] ; Anne Lebrun [France]Source :
- Genetica [ 0016-6707 ] ; 1999-06-01.
English descriptors
Abstract
Abstract: We have used internal coordinate molecular mechanics calculations to study how the DNA double helix deforms upon stretching. Results obtained for polymeric DNA under helical symmetry constraints suggest that two distinct forms, an unwound ribbon and a narrow fibre, can be formed as a function of which ends of the duplex are pulled. Similar results are also obtained with DNA oligomers. These experiments lead to force curves which exhibit a plateau as the conformational transition occurs. This behaviour is confirmed by applying an increasing force to DNA and observing a sudden length increase at a critical force value. It is finally shown some DNA binding proteins can also stretch DNA locally, to conformations related to those created by nanomanipulation.
Url:
DOI: 10.1023/A:1003776827836
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001246
- to stream Istex, to step Curation: 001246
- to stream Istex, to step Checkpoint: 001182
- to stream Main, to step Merge: 003974
- to stream Main, to step Curation: 003929
- to stream Main, to step Exploration: 003929
- to stream France, to step Extraction: 000265
Links to Exploration step
ISTEX:8233AB1020F9C22F09CD4C94C7001B15EC63EFCALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Modelling DNA stretching for physics and biology</title>
<author><name sortKey="Lavery, Richard" sort="Lavery, Richard" uniqKey="Lavery R" first="Richard" last="Lavery">Richard Lavery</name>
</author>
<author><name sortKey="Lebrun, Anne" sort="Lebrun, Anne" uniqKey="Lebrun A" first="Anne" last="Lebrun">Anne Lebrun</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:8233AB1020F9C22F09CD4C94C7001B15EC63EFCA</idno>
<date when="1999" year="1999">1999</date>
<idno type="doi">10.1023/A:1003776827836</idno>
<idno type="url">https://api.istex.fr/ark:/67375/VQC-T2646TRZ-6/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001246</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001246</idno>
<idno type="wicri:Area/Istex/Curation">001246</idno>
<idno type="wicri:Area/Istex/Checkpoint">001182</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001182</idno>
<idno type="wicri:doubleKey">0016-6707:1999:Lavery R:modelling:dna:stretching</idno>
<idno type="wicri:Area/Main/Merge">003974</idno>
<idno type="wicri:Area/Main/Curation">003929</idno>
<idno type="wicri:Area/Main/Exploration">003929</idno>
<idno type="wicri:Area/France/Extraction">000265</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Modelling DNA stretching for physics and biology</title>
<author><name sortKey="Lavery, Richard" sort="Lavery, Richard" uniqKey="Lavery R" first="Richard" last="Lavery">Richard Lavery</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biochimie Théorique, Institut de Biologie Physico‐Chimique, 13, rue Pierre et Marie Curie, 75005, Paris</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lebrun, Anne" sort="Lebrun, Anne" uniqKey="Lebrun A" first="Anne" last="Lebrun">Anne Lebrun</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biochimie Théorique, Institut de Biologie Physico‐Chimique, 13, rue Pierre et Marie Curie, 75005, Paris</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Genetica</title>
<title level="j" type="sub">An International Journal of Genetics and Evolution</title>
<title level="j" type="abbrev">Genetica</title>
<idno type="ISSN">0016-6707</idno>
<idno type="eISSN">1573-6857</idno>
<imprint><publisher>Kluwer Academic Publishers</publisher>
<pubPlace>Dordrecht</pubPlace>
<date type="published" when="1999-06-01">1999-06-01</date>
<biblScope unit="volume">106</biblScope>
<biblScope unit="issue">1-2</biblScope>
<biblScope unit="page" from="75">75</biblScope>
<biblScope unit="page" to="84">84</biblScope>
</imprint>
<idno type="ISSN">0016-6707</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0016-6707</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>conformational transitions</term>
<term>molecular mechanics</term>
<term>nanomanipulation</term>
<term>protein–DNA complexes</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: We have used internal coordinate molecular mechanics calculations to study how the DNA double helix deforms upon stretching. Results obtained for polymeric DNA under helical symmetry constraints suggest that two distinct forms, an unwound ribbon and a narrow fibre, can be formed as a function of which ends of the duplex are pulled. Similar results are also obtained with DNA oligomers. These experiments lead to force curves which exhibit a plateau as the conformational transition occurs. This behaviour is confirmed by applying an increasing force to DNA and observing a sudden length increase at a critical force value. It is finally shown some DNA binding proteins can also stretch DNA locally, to conformations related to those created by nanomanipulation.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Île-de-France</li>
</region>
<settlement><li>Paris</li>
</settlement>
</list>
<tree><country name="France"><region name="Île-de-France"><name sortKey="Lavery, Richard" sort="Lavery, Richard" uniqKey="Lavery R" first="Richard" last="Lavery">Richard Lavery</name>
</region>
<name sortKey="Lebrun, Anne" sort="Lebrun, Anne" uniqKey="Lebrun A" first="Anne" last="Lebrun">Anne Lebrun</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000265 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000265 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= France |étape= Analysis |type= RBID |clé= ISTEX:8233AB1020F9C22F09CD4C94C7001B15EC63EFCA |texte= Modelling DNA stretching for physics and biology }}
This area was generated with Dilib version V0.6.33. |